Acetate kinase (EC 2.7.2.1), primarily found in microorganisms, plays a crucial role in converting acetate into acetyl-CoA by phosphorylating acetate in the presence of ATP and a divalent cation. This enzyme is instrumental in the glycolytic pathway, and its levels increase when excess glucose is available. Studies have shown that bacterial mutants lacking acetate kinase experience inhibited growth in the presence of glucose, indicating the enzyme's role in managing and excreting surplus carbohydrates.
Alfa Chemistry offers high-quality acetate kinase, which is briefly purified from engineered E. coli, ensuring a reliable and efficient enzyme for research purposes.
Product Detail
| Product Name | Acetate Kinase (Crude Enzyme) |
|---|
| Catalog Number | BIOS9027423 |
| CAS Number | 9027-42-3 |
| Enzyme Commission Number | EC 2.7.2.1 |
| Source | E. Coli |
| Appearance | Clear to translucent yellow solution |
| Enzyme Activity | Undetermined |
| Package | 100 mL |
| Enzymatic Reaction | ATP + acetate = ADP + acetyl phosphate |
| Synonyms | Acetokinase; AckA; AK; acetic kinase; acetate kinase (phosphorylating) |
| Systematic Name | ATP: acetate phosphotransferase |
| Cofactor(s) | ATP |
| Application | Synthesis; industry |
| Storage | Store at -20 °C or lower, for at least 1 month. |
Biological Role
- Acetyl Phosphate Formation: The product, acetyl phosphate, is an important intermediate in metabolic pathways. It can serve as a phosphate donor in the synthesis of ATP from ADP in certain organisms, a process crucial for energy production.
- Energy Metabolism: In bacteria, acetate kinase is involved in the pathway that converts acetyl-CoA to acetate, a process important for generating ATP under anaerobic conditions.
- Regulation of Gene Expression: Acetyl phosphate, the product of the reaction catalyzed by acetate kinase, can also act as a signaling molecule, influencing the activity of regulatory proteins involved in gene expression.
Applications
- Biotechnology: Acetate kinase can be used in biotechnology applications for the production of acetyl phosphate, which is useful in various biochemical assays.
- Research: Studying acetate kinase can provide insights into metabolic pathways, energy production, and the regulation of gene expression, particularly in prokaryotes.
Choose Alfa Chemistry
Alfa Chemistry is committed to offering an extensive array of biological enzymes, backed by many years of expertise in biochemical research and enzyme production. With a strong foundation in the field, Alfa Chemistry is equipped to deliver top-tier products and technical support tailored to your research requirements. Opting for Alfa Chemistry for your acetate kinase needs guarantees superior quality, expert knowledge, and dedicated support for your scientific endeavors.
Our products and services are for research use only.
