Aspartate aminotransferase (AST), also known as Aspartate Aminotransferase or Glutamate Oxaloacetate Transaminase (GOT), is an enzyme that plays a crucial role in amino acid metabolism. AST is a pyridoxal phosphate (PLP)-dependent transaminase enzyme, classified under EC 2.6.1.1. It catalyzes the reversible transfer of an α-amino group between aspartate and glutamate. This essential function in amino acid metabolism makes AST a key player in various metabolic pathways. The enzyme was first characterized by Arthur Karmen and his colleagues in 1954. AST is predominantly found in tissues with high metabolic activity, including the liver, heart, skeletal muscle, kidneys, brain, and red blood cells.
Clinically, the measurement of serum AST levels, along with serum alanine transaminase (ALT) levels, is a standard diagnostic tool to assess liver health. The ratio of AST to ALT (AST/ALT ratio) is also frequently used as a biomarker to aid in diagnosing liver conditions. Elevated levels of these enzymes in the serum can indicate liver damage or other pathological conditions, making them valuable indicators in clinical diagnostics.
Alfa Chemistry offers high-quality aspartate transaminase that is briefly purified from engineered E. coli.
Product Detail
| Product Name | Aspartate Transaminase (Crude Enzyme) |
|---|
| Catalog Number | BIOS9000979 |
| CAS Number | 9000-97-9 |
| Enzyme Commission Number | EC 2.6.1.1 |
| Source | E. Coli |
| Appearance | Clear to translucent yellow solution |
| Enzyme Activity | Undetermined |
| Package | 100 mL |
| Enzymatic Reaction | L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate |
| Synonyms | Glutamic-oxaloacetic transaminase; glutamic-aspartic transaminase; transaminase A; 2-oxoglutarate-glutamate aminotransferase; glutamate oxaloacetate transaminase; aspartate aminotransferase |
| Systematic Name | L-aspartate:2-oxoglutarate aminotransferase |
| Cofactor(s) | - |
| Application | Synthesis; medicine; biotechnology |
| Storage | Store at -20 °C or lower, for at least 1 month. |
Choose Alfa Chemistry
For top-quality aspartate transaminase at competitive prices, choose Alfa Chemistry as your trusted supplier. We are committed to providing reliable products and exceptional customer service for a smooth purchasing experience. Contact us today to learn how our aspartate transaminase can benefit your research or industrial processes.
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