Catalase is an enzyme that is found in nearly all living organisms exposed to oxygen, including bacteria, plants, and animals. Its primary function is to catalyze the decomposition of hydrogen peroxide into water and oxygen, a critical reaction that protects cells from oxidative damage by reactive oxygen species (ROS). Catalase is a tetrameric enzyme, meaning it consists of four subunits. Each subunit contains a heme group at its active site, which plays a crucial role in the enzyme's ability to catalyze the breakdown of hydrogen peroxide. The heme group contains an iron ion, which is essential for the catalytic activity of catalase.
Alfa Chemistry offers high-quality catalase that is briefly purified from engineered E. coli.
Product Detail
| Product Name | Catalase (Crude Enzyme) |
|---|
| Catalog Number | BIOS9001052 |
| CAS Number | 9001-05-2 |
| Enzyme Commission Number | EC 1.11.1.6 |
| Source | E. coli |
| Appearance | Clear to translucent yellow solution |
| Package | 100 mL |
| Activity | Undetermined |
| Applications | Medicine; synthesis; analysis; biotechnology; industry |
| Storage | Store at -20 °C or lower, for at least 1 month. |
| Notes | Custom production can be done to your specifications. |
Mechanism of Action
The catalytic cycle of catalase involves two distinct but interrelated reactions:
- First Stage - Peroxidatic Reaction: The heme iron in catalase reacts with hydrogen peroxide, oxidizing the iron and creating water and a hydroperoxide intermediate.
- Second Stage - Catalatic Reaction: The hydroperoxide intermediate reacts with a second hydrogen peroxide molecule, which results in the formation of water and oxygen, and the regeneration of the original heme enzyme.
Applications
- Diagnostic Uses: Catalase activity can be measured in clinical laboratories and can serve as a diagnostic marker. For example, low levels of catalase activity are observed in individuals with acatalasia, a rare inherited disorder.
- Therapeutic Potential: Due to its protective role against oxidative damage, catalase is being investigated for its potential in therapies against diseases involving oxidative stress, including its application in some formulations of antioxidant supplements.
- Biotechnological Applications: Catalase is used in various industrial applications, including the removal of hydrogen peroxide from milk prior to cheese production and in the textile industry for removing hydrogen peroxide from fabrics after bleaching.
Choose Alfa Chemistry
Choose Alfa Chemistry for top-tier catalase and bespoke product customization tailored to your unique requirements. Our premium-quality enzymes are ideal for diverse applications, including biosensors and diagnostic assays. Contact us today to explore our customizable options and discover how we can supply the enzymes you need for your research and development endeavors.
Our products and services are for research use only.
