Choline kinase (EC 2.7.1.32) is an enzyme that plays a critical role in the metabolism of phospholipids, which are essential components of cell membranes. It catalyzes the first step in the biosynthesis of phosphatidylcholine, a major phospholipid in cell membranes, by phosphorylating choline to form phosphocholine. The enzyme's activity is crucial in various biological processes, including membrane synthesis, lipid signaling, and cellular growth.
Alfa Chemistry offers high-quality choline kinase that is briefly purified from engineered E. coli.
Product Detail
| Product Name | Choline Kinase (Crude Enzyme) |
|---|
| Catalog Number | BIOS9026679 |
| CAS Number | 9026-67-9 |
| Enzyme Commission Number | EC 2.7.1.32 |
| Source | E. Coli |
| Appearance | Clear to translucent yellow solution |
| Enzyme Activity | Undetermined |
| Package | 100 mL |
| Enzymatic Reaction | ATP + choline = ADP + phosphocholine |
| Synonyms | Choline phosphokinase; choline-ethanolamine kinase |
| Systematic Name | ATP:choline phosphotransferase |
| Cofactor(s) | ATP |
| Application | Biotechnology; drug development; diagnostics; medicine |
| Storage | Store at -20 °C or lower, for at least 1 month. |
Biological Significance
- Membrane Synthesis: By producing phosphocholine, choline kinase contributes to the synthesis of phosphatidylcholine, which is necessary for the formation and maintenance of cellular membranes.
- Signal Transduction: Phosphocholine itself is involved in cellular signaling pathways, influencing processes such as cell proliferation, apoptosis, and differentiation.
- Lipid Metabolism: Choline kinase activity is tightly linked to lipid metabolism, particularly in the liver where choline-derived metabolites are crucial for lipid transport and lipoprotein synthesis.
Research Applications
- Oncology Therapeutic Target: Overexpression or dysregulation of choline kinase is associated with various diseases, particularly cancer, making it a promising therapeutic target in oncology. Its involvement in cancer and other conditions has spurred interest in developing inhibitors to disrupt phospholipid metabolism and reduce tumor growth. Research is currently focused on choline kinase inhibitors for their potential in cancer treatment.
- Metabolic and Signaling Research: Choline kinase is also used in research as a marker for studying cell metabolism and signaling pathways. By measuring choline kinase activity or phosphocholine levels, researchers can gain insights into the metabolic state of cells, the effects of drugs on lipid metabolism, and the mechanisms underlying various diseases.
Choose Alfa Chemistry
Alfa Chemistry is committed to offering an extensive array of biological enzymes, backed by many years of expertise in biochemical research and enzyme production. With a strong foundation in the field, Alfa Chemistry is equipped to deliver top-tier products and technical support tailored to your research requirements. Opting for Alfa Chemistry for your choline kinase needs guarantees superior quality, expert knowledge, and dedicated support for your scientific endeavors.
Our products and services are for research use only.
