Cyclodextrin glucanotransferase (CGTase), identified by the Enzyme Commission (EC) number 2.4.1.19, is a pivotal enzyme involved in the transformation of starch and similar polysaccharides into cyclodextrins. In the field of enzymology, CGTase facilitates the cyclization of segments of 1,4-alpha-D-glucan molecules by establishing a 1,4-alpha-D-glucosidic bond. This enzyme is a member of the glycosyltransferase family, with a specific classification under hexosyltransferases. Its ability to produce cyclodextrins, which have numerous industrial and pharmaceutical applications, underscores its importance in biochemical processes.
Alfa Chemistry offers high-quality cyclodextrin glucanotransferase that is briefly purified from engineered E. coli.
Product Detail
| Product Name | Cyclodextrin Glucanotransferase (Crude Enzyme) |
|---|
| Catalog Number | BIOS9030095 |
| CAS Number | 9030-09-5 |
| Enzyme Commission Number | EC 2.4.1.19 |
| Source | E. Coli |
| Appearance | Clear to translucent yellow solution |
| Enzyme Activity | Undetermined |
| Package | 100 mL |
| Enzymatic Reaction | Cyclizes part of a (1→4)-α-D-glucan chain by formation of a (1→4)-α-D-glucosidic bond |
| Synonyms | Bacillus macerans amylase; cyclomaltodextrin glucotransferase; α-1,4-glucan 4-glycosyltransferase, cyclizing; 1,4-α-D-glucan 4-α-D-(1,4-α-D-glucano)-transferase (cyclizing) |
| Systematic Name | (1→4)-α-D-glucan:(1→4)-α-D-glucan 4-α-D-[(1→4)-α-D-glucano]-transferase (cyclizing) |
| Cofactor(s) | - |
| Application | Industry; synthesis; nutrition; pharmacology |
| Storage | Store at -20 °C or lower, for at least 1 month. |
Molecular Mechanism
CGTase catalyzes the conversion of starch into cyclodextrins through a few primary activities:
- Cyclization Reaction: CGTase cleaves a segment of a linear α-1,4-glucan chain and connects the ends to form a circular structure, i.e., a cyclodextrin.
- Coupling Reaction: It can also transfer a cyclodextrin ring onto a different linear α-1,4-glucan chain.
- Disproportionation Reaction: This involves the transfer of a segment of one starch molecule onto another.
- Hydrolysis Reaction: Though not its primary function, CGTase can also hydrolyze α-1,4 glycosidic bonds in starch.
Types of Cyclodextrins
CGTase produces three main types of cyclodextrins, depending on the number of D-glucose units:
- Alpha-Cyclodextrin: Composed of six glucose units.
- Beta-Cyclodextrin: Composed of seven glucose units.
- Gamma-Cyclodextrin: Composed of eight glucose units.
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