D-amino acid oxidase (DAAO, EC 1.4.3.3) is a significant flavoprotein enzyme recognized for its ability to catalyze the oxidative deamination of D-amino acids into corresponding keto acids and ammonia. This enzyme, which requires flavin adenine dinucleotide (FAD) as a cofactor, exhibits high stereoisomeric selectivity and a broad substrate range, making it a valuable tool in various biotechnological applications.
Alfa Chemistry offers high-quality D-amino acid oxidase that is briefly purified from engineered E. coli.
Product Detail
| Product Name | D-Amino Acid Oxidase (Crude Enzyme) |
|---|
| Catalog Number | BIOS9000888 |
| CAS Number | 9000-88-8 |
| Enzyme Commission Number | EC 1.4.3.3 |
| Source | E. Coli |
| Appearance | Clear to translucent yellow solution |
| Enzyme Activity | Undetermined |
| Package | 100 mL |
| Enzymatic Reaction | a D-amino acid + H2O + O2 = a 2-oxo carboxylate + NH3 + H2O2 |
| Synonyms | Ophio-amino-acid oxidase; L-amino acid:O2 oxidoreductase |
| Systematic Name | D-amino-acid:oxygen oxidoreductase (deaminating) |
| Cofactor(s) | FAD; flavin |
| Application | Biotechnology; industry; drug development; analysis; diagnostics; synthesis |
| Storage | Store at -20 °C or lower, for at least 1 month. |
Catalytic Mechanism
The enzymatic action of DAAO involves three main steps:
- Oxidation of D-Amino Acid: The amino group of the D-amino acid is oxidized by DAAO, forming an imino acid and reducing FAD to FADH2.
- Reoxidation of FADH2: In the presence of oxygen, FADH2 is reoxidized to FAD, producing hydrogen peroxide.
- Hydrolysis of Imino Acid: The imino acid is hydrolyzed to yield a keto acid and ammonia.
Industrial Applications
One of the most notable applications of DAAO is in the biocatalytic production of 7-aminocephalosporanic acid (7-ACA), a core structure for various cephalosporin antibiotics. The process involves two enzymatic steps:
- Oxidative Deamination: DAAO catalyzes the conversion of cephalosporin C (CPC) to glutaryl-7-aminocephalosporanic acid (GL-7-ACA) via an intermediate, α-Ketoacid-adipoyl-7-aminocephalosporanic acid (AKA-7-ACA).
- Hydrolysis: GL-7-ACA acylase then hydrolyzes the glutaryl side chain of GL-7-ACA, yielding the final product, 7-ACA.
Choose Alfa Chemistry
Alfa Chemistry is a trusted supplier of biocatalytic enzymes, delivering high-quality products at a competitive price/performance ratio. If you are in search of D-amino acid oxidase, look no further than Alfa Chemistry. We guarantee the quality and reliability of our products and offer exceptional customer service and support to ensure a seamless and efficient purchasing experience. Contact us today to learn more about our D-amino acid oxidase and discover how it can enhance your research.
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