Formate dehydrogenase (FDH), classified under EC 1.2.1.2, is an enzyme that catalyzes the oxidation of formate to carbon dioxide. FDH typically contains a molybdenum (Mo) or tungsten (W) cofactor at its active site, along with selenocysteine or cysteine residues. The enzyme exists in various forms across different species, including bacteria, archaea, and eukaryotes. The active site architecture allows the enzyme to facilitate the transfer of electrons from formate to NAD+, forming NADH.
Alfa Chemistry offers high-quality formate dehydrogenase that is briefly purified from engineered E. coli.
Product Detail
| Product Name | Formate Dehydrogenase (Crude Enzyme) |
|---|
| Catalog Number | BIOS9028857 |
| CAS Number | 9028-85-7 |
| Enzyme Commission Number | EC 1.2.1.2 |
| Source | E. coli |
| Appearance | Clear to translucent yellow solution |
| Package | 100 mL |
| Activity | Undetermined |
| Reaction | Formate + NAD+ = CO2 + NADH + H+ |
| Applications | Synthesis; biotechnology; medicine; analysis |
| Storage | Store at -20 °C or lower, for at least 1 month. |
| Notes | Custom production can be done upon request. |
Biological Role
FDH plays a crucial role in the microbial metabolism of one-carbon compounds. In bacteria, they are often involved in anaerobic respiration, allowing the organisms to utilize formate as an electron donor. In some organisms, FDH is part of the formate hydrogenlyase complex, which facilitates the conversion of formate into hydrogen and CO2 under anaerobic conditions.
Applications
- Biocatalysis: The enzyme can be used in used in enzymatic reduction processes, including the regeneration of NADH in industrial biocatalysis.
- Biosensors: Formate dehydrogenase can be utilized in biosensors to detect formate levels in various samples, which is useful in environmental monitoring and clinical diagnostics.
- Bioremediation: The enzyme's ability to convert formate to CO2 can be harnessed in bioremediation processes to treat formate-contaminated environments.
- Research and Engineering: Recent research has focused on engineering formate dehydrogenase to enhance its stability, activity, and substrate specificity. These efforts aim to improve the enzyme's performance in industrial applications and expand its utility in synthetic biology.
Choose Alfa Chemistry
Alfa Chemistry is a reliable supplier of biocatalytic enzymes, providing high-quality formate dehydrogenase. We offer custom production upon request, allowing for tailored solutions to meet individual research needs. We provide excellent customer service and support, ensuring a smooth and efficient purchasing experience. Contact us today to learn more about our formate dehydrogenase and how it can benefit your research.
Our products and services are for research use only.
