Inorganic diphosphatase, also known as pyrophosphatase, is a type of hydrolase enzyme that catalyzes the hydrolysis of inorganic pyrophosphate (PPi) into two inorganic phosphate (Pi) molecules. This reaction is important in many cellular processes as it helps to drive reactions that produce PPi as a byproduct by continuously removing PPi and thus preventing its accumulation.
Alfa Chemistry offers high-quality inorganic diphosphatase, which is briefly purified from engineered E. coli, ensuring a reliable and efficient enzyme for research purposes.
Product Detail
| Product Name | Inorganic Diphosphatase (Crude Enzyme) |
|---|
| Catalog Number | BIOS9024822 |
| CAS Number | 9024-82-2 |
| Enzyme Commission Number | EC 3.6.1.1 |
| Source | E. Coli |
| Appearance | Clear to translucent yellow solution |
| Enzyme Activity | Undetermined |
| Package | 100 mL |
| Enzymatic Reaction | Diphosphate + H2O = 2 phosphate |
| Systematic Name | Diphosphate phosphohydrolase |
| Application | Molecular biology; synthesis; drug development |
| Storage | Store at -20 °C or lower, for at least 1 month |
Function and Importance
Function and Importance
The hydrolysis of PPi is energetically favorable and often coupled with biosynthetic reactions, such as DNA, RNA, and protein synthesis, in order to drive these processes forward. By converting PPi into two Pi, the inorganic diphosphatase enzyme ensures that these reactions are pulled in the direction of product formation.
Biosynthesis
In the context of nucleic acid metabolism, the synthesis of polymers like DNA and RNA releases PPi as a byproduct. The removal of PPi by inorganic diphosphatase ensures that nucleotide polymerization is more efficient and less likely to reverse.
Mineralization
In prokaryotes and eukaryotes, phosphate availability is critical. In tissues like bones and teeth, the localized generation of Pi from PPi hydrolysis might play a role in the mineralization processes.
Signal Transduction
In some cases, PPi can act as a signal molecule. Regulating its concentration through diphosphatase activity helps maintain appropriate signal transduction pathways.
Genetic and Biotechnological Applications
- Gene Regulation: The genes encoding inorganic diphosphatase can be regulated to up or downscale the production of the enzyme in biotechnological applications where biosynthetic efficiency is paramount, such as in the production of pharmaceutically relevant compounds.
- Genetic Engineering: Through genetic engineering, microbes can be optimized for higher yielding biosynthesis pathways by manipulating the expression of inorganic diphosphatase to handle increased flux through pathways that produce PPi.
Choose Alfa Chemistry
Alfa Chemistry is dedicated to offering an extensive array of biological enzymes, underpinned by extensive research and production expertise in biochemistry. With a robust foundation in the industry, Alfa Chemistry is always prepared to deliver high-quality products and professional technical support to meet your requirements. For more information about our inorganic diphosphatase products, please contact us.
Our products and services are for research use only.
