L-lactate dehydrogenase (LDH or LD) is an enzyme present in nearly all living cells, including those of animals, plants, and prokaryotes. It plays a crucial role in catalyzing the conversion of lactate to pyruvic acid and vice versa, while also converting NAD+ to NADH and back. As a dehydrogenase, LDH facilitates the transfer of a hydride ion from one molecule to another. This enzyme is widely expressed in various body tissues, such as blood cells and heart muscle. Due to its release during tissue damage, LDH serves as a biomarker for common injuries and diseases, including heart failure.
Alfa Chemistry offers high-quality L-lactate dehydrogenase that is briefly purified from engineered E. coli.
Product Detail
| Product Name | L-Lactate Dehydrogenase (Crude Enzyme) |
|---|
| Catalog Number | BIOS9001609 |
| CAS Number | 9001-60-9 |
| Enzyme Commission Number | EC 1.1.1.27 |
| Source | E. coli |
| Appearance | Clear to translucent yellow solution |
| Package | 100 mL |
| Activity | Undetermined |
| Applications | Medicine; synthesis; biotechnology; drug development. |
| Storage | Store at -20 °C or lower, for at least 1 month. |
| Notes | Custom production can be done to your specifications. |
Function
LDH catalyzes the following reversible reaction:
Pyruvate + NADH + H+ ⇌ Lactate + NAD+
In the absence of oxygen, cells rely on anaerobic glycolysis for ATP production. During this process, pyruvate is reduced to lactate by LDH, regenerating NAD+ from NADH. This regeneration of NAD+ is essential for glycolysis to continue, allowing the cell to produce ATP anaerobically.
Enzyme Kinetics and Regulation
LDH activity is regulated by:
- Substrate Availability: Concentrations of pyruvate and NADH.
- Product Inhibition: Accumulation of lactate can inhibit LDH activity.
- Allosteric Regulation: In some organisms, LDH can be regulated by allosteric effectors.
Research and Industrial Applications
- Biotechnology: LDH is used in various diagnostic assays and biotechnological applications to monitor metabolic processes.
- Pharmaceuticals: Targeting LDH is explored in cancer therapy to disrupt the altered metabolic pathways in cancer cells.
- Sports Science: LDH levels are measured to assess muscle damage and recovery in athletes.
Choose Alfa Chemistry
Unlock the potential of your research and industrial applications with Alfa Chemistry's high-quality L-lactate dehydrogenase. Tailor-made to meet your exact specifications, our customizable products deliver precise results for all your needs. Contact us today to discover how our L-lactate dehydrogenase can drive your success and innovation.
Our products and services are for research use only.
