L-phenylalanine dehydrogenase (EC 1.4.1.20), often abbreviated as PheDH, is an enzyme that plays a key role in the metabolism of amino acids, specifically phenylalanine. This enzyme catalyzes the oxidative deamination of L-phenylalanine to phenylpyruvate, using NAD+ as a cofactor.
Alfa Chemistry offers high-quality L-phenylalanine dehydrogenase that is briefly purified from engineered E. coli.
Product Detail
| Product Name | L-Phenylalanine Dehydrogenase (Crude Enzyme) |
|---|
| Catalog Number | BIOS69403129 |
| CAS Number | 69403-12-9 |
| Enzyme Commission Number | EC 1.4.1.20 |
| Source | E. coli |
| Appearance | Clear to translucent yellow solution |
| Package | 100 mL |
| Enzymatic Reaction | L-phenylalanine + H2O + NAD+ = phenylpyruvate + NH3 + NADH + H+ |
| Systematic Name | L-phenylalanine:NAD+ oxidoreductase (deaminating) |
| Cofactor(s) | NAD+; NADH |
| Applications | Analysis; biotechnology; medicine; synthesis |
| Storage | Store at -20 °C or lower, for at least 1 month. |
Applications
L-phenylalanine dehydrogenase has diverse and significant applications across various fields, particularly in biochemistry, medicine, and industrial biotechnology. Here are more details about its applications:
Diagnostic Applications
- Phenylketonuria (PKU) Screening: One of the most important clinical applications of PheDH is in the diagnosis of phenylketonuria, a genetic disorder characterized by the inability to metabolize phenylalanine. Elevated levels of phenylalanine can lead to severe intellectual disabilities if left untreated. PheDH-based assays are used in newborn screening programs to detect elevated phenylalanine levels in blood samples.
Biocatalysis in Organic Synthesis
- Synthesis of L-Phenylalanine Derivatives: PheDH can be used to produce enantiomerically pure L-phenylalanine derivatives, which are valuable in pharmaceutical synthesis. The enzyme's stereospecificity makes it an excellent tool for generating chiral compounds.
- Production of Aromatic Amino Acids: PheDH can be employed in the biosynthesis of various aromatic amino acids through deamination processes, facilitating the production of phenylalanine derivatives used in pharmaceuticals and nutraceuticals.
Industrial Biotechnology
- Phenylalanine Biosensors: PheDH is used in the development of biosensors for detecting phenylalanine concentrations in various samples. These biosensors can be applied in medical diagnostics, environmental monitoring, and food quality control.
- Enzyme-Based Analytical Devices: These devices leverage the specificity of PheDH to provide accurate and reliable measurements of phenylalanine in complex mixtures.
- Strain Improvement: Microorganisms engineered to overexpress PheDH can be used in fermentation processes to enhance the production of phenylalanine and its derivatives. This is particularly useful in the large-scale production of amino acids for food additives and pharmaceuticals.
Research Applications
- Pathway Elucidation: PheDH is used in research to study the metabolic pathways involving phenylalanine. By examining the enzyme's activity, researchers can gain insights into the regulation and interaction of metabolic networks.
- Enzyme Kinetics: PheDH serves as a model enzyme for studying dehydrogenase activity, enzyme kinetics, and mechanisms of catalysis. Research in this area can lead to the development of new enzymes with enhanced properties for industrial applications.
Choose Alfa Chemistry
Alfa Chemistry is a reliable supplier of biocatalytic enzymes, offering high-quality L-phenylalanine dehydrogenase. We offer custom production services to provide tailored solutions that meet the specific needs of your research. We are committed to providing excellent customer service and support to ensure a smooth and efficient purchasing experience. Contact us today to learn more about our L-phenylalanine dehydrogenase and discover how it can benefit your research.
Our products and services are for research use only.
