NAD kinase (EC 2.7.1.23) is a pivotal enzyme in cellular metabolism, responsible for phosphorylating nicotinamide adenine dinucleotide (NAD+) to produce nicotinamide adenine dinucleotide phosphate (NADP+). This reaction is essential because NADP+, along with its reduced counterpart NADPH, is crucial in various biosynthetic (anabolic) pathways. These pathways include the synthesis of fatty acids, cholesterol, and nucleotides. The availability and balance of NADPH are fundamental for maintaining the redox state of the cell and supporting anabolic processes, making the role of NAD kinase indispensable.
Alfa Chemistry offers high-quality NAD kinase (crude enzyme) that is briefly purified from engineered E. coli.
Product Detail
| Product Name | NAD Kinase (Crude Enzyme) |
|---|
| Catalog Number | BIOS9032660 |
| CAS Number | 9032-66-0 |
| Enzyme Commission Number | EC 2.7.1.23 |
| Source | E. Coli |
| Appearance | Clear to translucent yellow solution |
| Enzyme Activity | Undetermined |
| Package | 100 mL |
| Enzymatic Reaction | ATP + NAD+ = ADP + NADP+ |
| Synonyms | DPN kinase; nicotinamide adenine dinucleotide kinase (phosphorylating); nicotinamide adenine dinucleotide kinase; NADK |
| Systematic Name | ATP:NAD+ 2'-phosphotransferase |
| Cofactor(s) | NAD+ |
| Application | Synthesis; agriculture; analysis |
| Storage | Store at -20 °C or lower, for at least 1 month. |
Structure
NAD kinase is generally a monomeric or dimeric protein, and its structure typically includes nucleotide-binding domains that interact with both ATP and NAD+. The enzyme's active site is well-conserved across species and often includes residues critical for catalysis, such as those involved in the binding of magnesium or other divalent metal ions, which are required as cofactors for the kinase activity.
Applications in Research and Industry
- Metabolic Engineering: Understanding and manipulating NAD kinase activity is of interest in metabolic engineering, where enhanced NADPH production can drive the synthesis of desirable compounds like biofuels or pharmaceuticals.
- Antibiotic Target: In bacteria, NAD kinase is essential for survival, making it a potential target for developing new antibiotics.
- Plant Biology: In plants, NAD kinase is involved in photosynthesis and stress responses, making it a focus of research in agriculture and plant biotechnology.
Choose Alfa Chemistry
For top-quality NAD kinase at competitive prices, choose Alfa Chemistry as your trusted supplier. We are committed to providing reliable products and exceptional customer service for a smooth purchasing experience. Contact us today to learn how our NAD kinase can benefit your research or industrial processes.
Our products and services are for research use only.
