Nucleoside-diphosphate kinases (NDPKs), also known as NDP Kinase, (poly)nucleotide kinases, and nucleoside diphosphokinases, are enzymes that catalyze the reversible exchange of terminal phosphate groups between different nucleoside diphosphates (NDPs) and nucleoside triphosphates (NTPs). This catalytic activity enables the production of various nucleotide triphosphates. In this process, NDPs serve as phosphate group acceptors, while NTPs act as donors.
Beyond their primary catalytic function, NDPKs are also involved in a range of cellular processes, including cell proliferation, differentiation, and development. They are integral to signal transduction pathways, the function of G protein-coupled receptors, endocytosis, and gene expression. This extensive involvement underscores the enzyme's significance in maintaining cellular and physiological homeostasis.
Alfa Chemistry offers high-quality nucleoside-diphosphate kinase, which is briefly purified from engineered E. coli, ensuring a reliable and efficient enzyme for research purposes.
Product Detail
| Product Name | Nucleoside-Diphosphate Kinase (Crude Enzyme) |
|---|
| Catalog Number | BIOS9026511 |
| CAS Number | 9026-51-1 |
| Enzyme Commission Number | EC 2.7.4.6 |
| Source | E. Coli |
| Appearance | Clear to translucent yellow solution |
| Enzyme Activity | Undetermined |
| Package | 100 mL |
| Enzymatic Reaction | ATP + nucleoside diphosphate = ADP + nucleoside triphosphate |
| Synonyms | Nucleoside 5'-diphosphate kinase; nucleoside diphosphate (UDP) kinase; UDP kinase; nucleotide phosphate kinase; nucleoside diphosphokinase; uridine diphosphate kinase |
| Systematic Name | ATP:nucleoside-diphosphate phosphotransferase |
| Cofactor(s) | ATP |
| Application | Medicine |
| Storage | Store at -20 °C or lower, for at least 1 month. |
Applications in Synthetic Biology
- Genome Engineering: When designing and synthesizing artificial genomes or large DNA/RNA constructs, ensuring a balanced supply of NTPs is crucial. NDPK helps maintain this balance, facilitating efficient nucleic acid synthesis.
- In vitro Transcription and Translation Systems: In cell-free systems used for protein synthesis, researcher-created nucleoside triphosphates are often needed. NDPK can be included to help recycle diphosphates back to triphosphates, enhancing reaction efficiency.
- Metabolic Engineering: In engineered metabolic pathways, especially those involving nucleotide-dependent enzymes, NDPK can help ensure that adequate levels of required NTPs are maintained, thus optimizing pathway functionality.
- Bioproduction Systems: For the industrial production of nucleotides or nucleotide derivatives, NDPK can be used to regenerate NTPs from NDPs, reducing costs and increasing yield.
Choose Alfa Chemistry
Alfa Chemistry is committed to offering an extensive array of biological enzymes, backed by many years of expertise in biochemical research and enzyme production. With a strong foundation in the field, Alfa Chemistry is equipped to deliver top-tier products and technical support tailored to your research requirements. Opting for Alfa Chemistry for your nucleoside-diphosphate kinase needs guarantees superior quality, expert knowledge, and dedicated support for your scientific endeavors.
Our products and services are for research use only.
