Valine dehydrogenase (EC 1.4.1.8) is an enzyme that catalyzes the oxidative deamination of L-valine to 3-methyl-2-oxobutanoate (α-ketoisovalerate). This enzyme plays a critical role in the metabolism of branched-chain amino acids (BCAAs) in various organisms, including bacteria, plants, and some animals. It is involved in the catabolism of valine, which is a key process for cellular energy production and the synthesis of other important biomolecules.
Alfa Chemistry offers high-quality valine dehydrogenase that is briefly purified from engineered E. coli.
Product Detail
| Product Name | Valine Dehydrogenase (Crude Enzyme) |
|---|
| Catalog Number | BIOS37255393 |
| CAS Number | 37255-39-3 |
| Enzyme Commission Number | EC 1.4.1.8 |
| Source | E. Coli |
| Appearance | Clear to translucent yellow solution |
| Enzyme Activity | Undetermined |
| Package | 100 mL |
| Enzymatic Reaction | L-valine + H2O + NADP+ = 3-methyl-2-oxobutanoate + NH3 + NADPH + H+ |
| Systematic Name | L-valine:NADP+ oxidoreductase (deaminating) |
| Cofactor(s) | NAD+; NADH; NADP+ |
| Storage | Store at -20 °C or lower, for at least 1 month. |
Importance in Research and Industry
Biotechnological Applications
- Industrial Enzyme Production: Due to its role in amino acid metabolism, valine dehydrogenase can be used in the production of keto acids and amines, which are valuable in the chemical and pharmaceutical industries.
- Metabolic Engineering: Understanding and manipulating the activity of valine dehydrogenase can help in engineering microbial strains for the overproduction of branched-chain amino acids and their derivatives.
Medical Research
- Disease Studies: Alterations in valine metabolism are linked to various metabolic disorders. Studying valine dehydrogenase can provide insights into conditions like maple syrup urine disease (MSUD) and other inborn errors of metabolism.
Agricultural Research
- Crop Improvement: Insights into the role of valine dehydrogenase in plants can lead to the development of crops with improved nutritional profiles or stress responses.
Experimental Studies
- Kinetic Studies: To determine the enzyme's catalytic efficiency, substrate specificity, and reaction mechanism.
- Structural Biology: Using X-ray crystallography or NMR spectroscopy to resolve the enzyme's 3D structure.
- Genetic Studies: Cloning and overexpression of the enzyme in model organisms to study its physiological roles and regulatory mechanisms.
Choose Alfa Chemistry
Alfa Chemistry is a trusted supplier of biocatalytic enzymes, delivering high-quality products at a competitive price/performance ratio. If you are in search of valine dehydrogenase, look no further than Alfa Chemistry. We guarantee the quality and reliability of our products and offer exceptional customer service and support to ensure a seamless and efficient purchasing experience. Contact us today to learn more about our valine dehydrogenase and discover how it can enhance your research.
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