Acetolactate synthase (ALS), also known as acetohydroxyacid synthase (AHAS), is an enzyme classified under EC 2.2.1.6. It plays a crucial role in the biosynthesis of branched-chain amino acids, specifically isoleucine, leucine, and valine. ALS catalyzes the first common step in the pathway leading to the production of these essential amino acids.
Alfa Chemistry offers high-quality acetolactate synthase that is briefly purified from engineered E. coli.
Product Detail
| Product Name | Acetolactate Synthase (Crude Enzyme) |
|---|
| Catalog Number | BIOS9027456 |
| CAS Number | 9027-45-6 |
| Enzyme Commission Number | EC 2.2.1.6 |
| Source | E. Coli |
| Appearance | Clear to translucent yellow solution |
| Enzyme Activity | Undetermined |
| Package | 100 mL |
| Enzymatic Reaction | 2 pyruvate = 2-acetolactate + CO2 |
| Synonyms | α-acetohydroxy acid synthetase; acetohydroxy acid synthetase; acetohydroxyacid synthase; acetolactate pyruvate-lyase (carboxylating) |
| Systematic Name | Pyruvate:pyruvate acetaldehydetransferase (decarboxylating) |
| Cofactor(s) | FAD; thiamine diphosphate |
| Application | Agriculture; biotechnology; drug development; synthesis; molecular biology |
| Storage | Store at -20 °C or lower, for at least 1 month. |
Regulation
ALS activity is tightly regulated through several mechanisms:
- Feedback Inhibition: The end products (isoleucine, leucine, and valine) can inhibit ALS activity to prevent overproduction.
- Post-translational Modifications: Phosphorylation and other modifications can affect enzyme activity.
- Gene Expression: Transcriptional regulation of ALS genes in response to environmental and metabolic signals.
Applications
- Herbicide Target: ALS is the target of several herbicides (e.g., sulfonylureas, imidazolinones). These herbicides inhibit ALS, preventing the synthesis of BCAAs, leading to plant death. The development of herbicide-resistant crops involves modifications in the ALS gene to confer resistance.
- Drug Development: ALS is a potential target for antibiotics development since it is essential in bacteria but absent in animals, providing a pathway to design selective antimicrobial agents.
Choose Alfa Chemistry
Alfa Chemistry is a trusted supplier of biocatalytic enzymes, delivering high-quality products at a competitive price/performance ratio. If you are in search of acetolactate synthase, look no further than Alfa Chemistry. We guarantee the quality and reliability of our products and offer exceptional customer service and support to ensure a seamless and efficient purchasing experience. Contact us today to learn more about our acetolactate synthase and discover how it can enhance your research.
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