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Alanine Dehydrogenase (Crude Enzyme)
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Alanine Dehydrogenase (Crude Enzyme)

Alanine dehydrogenase (EC 1.4.1.1) is an enzyme that plays a crucial role in amino acid metabolism, specifically in the reversible conversion of L-alanine to pyruvate and ammonia. This reaction is coupled with the reduction of NAD+ to NADH, which makes alanine dehydrogenase important for cellular redox balance and energy metabolism.

Alfa Chemistry offers high-quality alanine dehydrogenase that is briefly purified from engineered E. coli.

Product Detail

Product NameAlanine Dehydrogenase (Crude Enzyme)
Catalog NumberBIOS9029065
CAS Number9029-06-5
Enzyme Commission NumberEC 1.4.1.1
SourceE. coli
Enzymatic ReactionL-alanine + H2O + NAD+ = pyruvate + NH3 + NADH + H+
SynonymsAlaDH; L-alanine dehydrogenase; NAD-linked alanine dehydrogenase; α-alanine dehydrogenase; NAD-dependent alanine dehydrogenase; alanine oxidoreductase; NADH-dependent alanine dehydrogenase
Systematic NameL-alanine:NAD+ oxidoreductase (deaminating)
Cofactor(s)NAD+; NADH
ApplicationsSynthesis; biotechnology; nutrition
StorageStore at -20 °C or lower, for at least 1 month.

Biological Role

  • Amino Acid Metabolism: Alanine dehydrogenase is pivotal in the catabolism and synthesis of alanine. It helps maintain the balance of amino acids in the cell.
  • Nitrogen Metabolism: By producing ammonia (NH3), the enzyme contributes to nitrogen metabolism, which is essential for the synthesis of nucleotides and other nitrogenous compounds.
  • Energy Production: The conversion of L-alanine to pyruvate feeds into the Krebs cycle, thus aiding in cellular respiration and energy production.
  • Redox Balance: The production of NADH in the reaction can be used in the electron transport chain to produce ATP, thus playing a role in the cell's energy economy.

Applications

  • Industrial and Biotechnological: Alanine dehydrogenase is used in the biosynthesis of L-alanine, which is an important amino acid for various applications including pharmaceuticals, food additives, and supplements.
  • Research: It is used as a model enzyme to study amino acid metabolism and enzyme kinetics.
  • Clinical: Understanding the function and regulation of alanine dehydrogenase can provide insights into metabolic disorders related to amino acid imbalances.

Choose Alfa Chemistry

Alfa Chemistry is a reliable supplier of biocatalytic enzymes, offering high-quality alanine dehydrogenase. We offer custom production services to provide tailored solutions that meet the specific needs of your research. We are committed to providing excellent customer service and support to ensure a smooth and efficient purchasing experience. Contact us today to learn more about our alanine dehydrogenase and discover how it can benefit your research.

Our products and services are for research use only.